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Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model
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JoVE Revista Biología del desarrollo
Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model

Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model

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06:43 min

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October 09, 2018

DOI:

10.3791/58047-v

DOI:
10.3791/58047-v

•

06:43 min
October 09, 2018

•

5 Views
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1Division of General, Thoracic, and Fetal Surgery, Center for Fetal Research,Children’s Hospital of Philadelphia, 2Division of Plastic Reconstructive Surgery, Center for Fetal Research,Children’s Hospital of Philadelphia

Summary

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We describe a protocol for performing an in utero transplantation (IUT) through intravenous and intra-amniotic routes of injection in the murine model. This protocol can be used to introduce cells, viral vectors, and other substances into the unique immune-tolerant fetal environment.

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In Utero TransplantationMurine ModelEmbryologyGene TherapyStem Cell TransplantationIntravenousIntra-amnioticMicro-injectionPregnant MouseSurgical ProcedureLocal AnesthesiaFetal Exposure

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Cite this Article

Ahn, N. J., Stratigis, J. D., Coons, B. E., Flake, A. W., Nah-Cederquist, H., Peranteau, W. H. Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model. J. Vis. Exp. (140), e58047, doi:10.3791/58047 (2018).

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