Rivista
/
Biochimica
/
A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton
JoVE Journal
Biochimica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biochimica
A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton

A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton

DOI:
10.3791/58038-v

•

05:47 min

•

July 29, 2018

•

, ,
1Department of Chemical Engineering,University of Florida

Capitoli

  • 00:04Titolo
  • 00:47Preparing Cells for Imaging
  • 01:29Microscopy and Image Acquisition
  • 04:02Results: Characterization of the Nuclear Deformation
  • 05:29Conclusion

Summary

Traduzione automatica

Traduzione automatica

In this protocol, we describe a micropipette method to directly apply a controlled force to the nucleus in a living cell. This assay allows interrogation of nuclear mechanical properties in the living, adherent cell.

Tags

Nuclear MechanicsNuclear DeformationNucleo-cytoskeleton IntegrationNuclear Mechanical PropertiesAdherent CellsProbing ForceMicromanipulatorMicropipetteHutchinson-Gilford Progerial CellsNIH-3T3 Fibroblasts

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

A Colorimetric Method for Measuring Iron Content in Plants

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Imaging of Extracellular Vesicles by Atomic Force Microscopy

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Read Article