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单核苷酸延长和 MALDI 质谱分析校对及 DNA 修复方法
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
DOI:

11:08 min

June 19, 2018

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Capitoli

  • 00:05Titolo
  • 01:17Proofreading Reactions
  • 03:16Terminate the Reaction and Transfer Reaction Products to a Matrix Chip
  • 04:54Setup the Assay Information on the Mass Spectrometry
  • 06:18MALDI-TOF Mass Spectrometry (MS) Operation and Data Analysis
  • 08:00Results: DNA Proofreading and Repair Analyzed by MALDI-TOF MS
  • 09:49Conclusion

Summary

Traduzione automatica

采用高分辨率 MALDI 质谱和单核苷酸扩展策略, 建立了 dna 聚合酶校对和 dna 修复实验的非标记非射电同位素方法。试验证明是非常具体, 简单, 快速, 并易于执行校对和修复补丁短于 9-核苷酸。

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