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大人の皮膚組織から人間ケラチノ サイト主を特定する簡易で効率的な方法
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
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大人の皮膚組織から人間ケラチノ サイト主を特定する簡易で効率的な方法

9,698 Views

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06:17 min

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August 25, 2018

DOI:

10.3791/57784-v

DOI:
10.3791/57784-v

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06:17 min
August 25, 2018

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3 Views
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1Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology,Shandong University, 2Suzhou Institute of Shandong University, 3Department of Urology,Qilu Hospital of Shandong University

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Primary Human KeratinocytesIsolationCultureSimplified MethodEfficientEpidermal CellsLaboratory ApplicationsClinical ApplicationsHomogenizationEnzymatic DigestionTrypsinDNase ICell StrainerCell CultureROCK InhibitorKeratinocyte MediumPassaging

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Liu, Z., Wen, J., Leng, X., Zhou, Q., Zhou, C., Zhao, H., Wu, X. A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue. J. Vis. Exp. (138), e57784, doi:10.3791/57784 (2018).

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