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Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
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JoVE Journal Biochimica
Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue

DOI:
10.3791/57760-v

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06:18 min

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June 21, 2018

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, , , , , , , ,
1Department of Molecular, Cell and Developmental Biology,UCLA, 2Department of Biological Chemistry,David Geffen School of Medicine, 3Molecular Biology Institute Interdepartmental Program,UCLA

Capitoli

  • 00:04Titolo
  • 00:31Generate Slides of Frozen Mouse Skin
  • 01:53Slides and Staining Solution Preparation
  • 02:54Staining and Imaging
  • 04:08Results: Representative Results of Lactate Dehydrogenase Staining
  • 05:43Conclusion

Summary

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We describe a protocol for mapping the spatial distribution of enzymatic activity for enzymes that generate nicotinatmide adenine dinucleotide phosphate (NAD(P)H) + H+ directly in tissue samples.

Tags

Lactate DehydrogenaseEnzyme ActivityCryomoldCryosectioningHistochemical StainingNADPMSNitrotetrazolium Blue ChlorideLactateEnzyme Localization

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