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Isolatie, karakterisering en High Throughput extracellulaire Flux-analyse van muis primaire renale tubulaire epitheliale cellen
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June 20, 2018
DOI:
1Department of Molecular and Cellular Pharmacology,University of Miami Leonard M. Miller School of Medicine, 2Interdisciplinary Stem Cell Institute,University of Miami Leonard M. Miller School of Medicine, 3Department of Medicine, Division of Cardiology,University of Miami Leonard M. Miller School of Medicine, 4Vascular Biology Institute,University of Miami Leonard M. Miller School of Medicine, 5Peggy and Harold Katz Family Drug Discovery Center,University of Miami Leonard M. Miller School of Medicine
Summary
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Dit protocol biedt een rendabele aanpak om te isoleren en karakteriseren van muis primaire renale tubulaire cellen die vervolgens sub kunnen worden gekweekt om te beoordelen renale biologische functies- ex vivo, met inbegrip van de mitochondriale Bioenergetica.
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Ding, W., Yousefi, K., Shehadeh, L. A. Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells. J. Vis. Exp. (136), e57718, doi:10.3791/57718 (2018).
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