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Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain
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Neuroscienze
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JoVE Journal Neuroscienze
Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain

Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain

DOI:
10.3791/57391-v

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08:23 min

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May 12, 2018

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, , , , , , , , , ,
1Department of Anesthesiology,Ohio State University College of Medicine, 2Medical Student Research Program,Ohio State University College of Medicine, 3Department of Anesthesiology and Pain Medicine,Nationwide Children’s Hospital, 4Department of Neuroscience,University of Kentucky Medical Center

Capitoli

  • 00:04Titolo
  • 01:19Set Up for Surgery
  • 03:38Microelectrode Array Placement
  • 06:19Results: In Vivo Glutamate Measurements from the Piglet Hippocampus
  • 07:56Conclusion

Summary

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This study explores the novel use of enzyme-based microelectrode array (MEA) technology to monitor in vivo neurotransmitter activity in piglets. The hypothesis was that glutamate dysregulation contributes to the mechanism of anesthetic neurotoxicity. Here, we present a protocol to adapt MEA technology to study the mechanism of anesthesia-induced neurotoxicity.

Tags

Keywords: Microelectrode ArrayNeurotransmittersAnesthesia-induced NeurotoxicityPiglet BrainIn Vivo Glutamate ActivityPediatric Brain TraumaEpilepsyStrokePiglet ModelSevoflurane AnesthesiaStereotaxic FrameRocuroniumVital Signs Monitoring

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