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Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

DOI:
10.3791/57290-v

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09:38 min

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February 20, 2018

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, , , ,
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, 2UMR7104,Centre National de la Recherche Scientifique, 3U964,Institut National de la Santé et de la Recherche Médicale, 4Université de Strasbourg

Capitoli

  • 00:04Titolo
  • 00:53Preparing Molds and Mounting Agarose and Embedding Embryos
  • 03:31Photoconversion
  • 05:12Unmounting Photoconverted Embryos
  • 06:20Imaging Photoconverted Cells at Later Embryonic Stages
  • 08:05Results: Photoconversion of Kaede Fluorescent Protein in Zebrafish Embryonic Endocardial Cells
  • 09:01Conclusion

Summary

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This protocol describes a method for the photoconversion of Kaede fluorescent protein in endocardial cells of the living zebrafish embryo that enables the tracking of endocardial cells during atrioventricular canal and atrioventricular heart valve development.

Tags

Cell PhotoconversionZebrafish EmbryoAtrioventricular CanalHeart Valve FormationCardiac DevelopmentMutantsTime-lapse MicroscopyAgaroseMounting MediumLow Melting Point AgaroseTricaineBDM

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