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Isolating Lymphocytes from the Mouse Small Intestinal Immune System
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Isolating Lymphocytes from the Mouse Small Intestinal Immune System
Capitoli
- 00:04Titolo
- 01:00Isolation of the Mesenteric Lymph Nodes, Intestines, and Peyer’s Patches
- 03:45Isolation of Lymphocytes from the Inductive Sites
- 04:22Isolation of Intraepithelial Lymphocytes from the Small Intestine
- 07:28Isolation of Lamina Propria Lymphocytes from the Small Intestine
- 09:39Results: Characterization of Isolated Lymphocytes
- 10:52Conclusion
Here we describe a detailed protocol for the isolation of lymphocytes from the inductive sites including the gut-associated lymphoid tissue Peyer's patches and the draining mesenteric lymph nodes, and the effector sites including the lamina propria and the intestinal epithelium of the small intestinal immune system.
