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साइट उत्पंन करने के लिए एक सतही प्रोटोकॉल-विशेष रूप से ई कोलाई में Acetylated प्रोटीन
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JoVE Journal Immunologia e infezioni
A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
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साइट उत्पंन करने के लिए एक सतही प्रोटोकॉल-विशेष रूप से ई कोलाई में Acetylated प्रोटीन

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11:08 min

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December 09, 2017

DOI:

10.3791/57061-v

DOI:
10.3791/57061-v

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11:08 min
December 09, 2017

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6 Views
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1Department of Chemistry and Biochemistry,University of Arkansas, 2Cell and Molecular Biology Program,University of Arkansas, 3Department of Biological Sciences,University of Arkansas, 4Fayetteville High School

Tags

Site-specific AcetylationProtein AcetylationGenetic Code ExpansionPyrrolysyl-tRNA SynthetaseAmber Stop CodonE. Coli ExpressionSite-directed MutagenesisAcetyllysine Incorporation

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Venkat, S., Gregory, C., Meng, K., Gan, Q., Fan, C. A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli. J. Vis. Exp. (130), e57061, doi:10.3791/57061 (2017).

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