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在大肠杆菌中生成特异的乙酰蛋白的简易协议
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
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在大肠杆菌中生成特异的乙酰蛋白的简易协议

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11:08 min

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December 09, 2017

DOI:

10.3791/57061-v

DOI:
10.3791/57061-v

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11:08 min
December 09, 2017

•

1 Views
, , , ,
1Department of Chemistry and Biochemistry,University of Arkansas, 2Cell and Molecular Biology Program,University of Arkansas, 3Department of Biological Sciences,University of Arkansas, 4Fayetteville High School

Summary

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基因编码扩展是研究广泛的生物过程, 包括蛋白质乙酰化的有力工具。在这里, 我们演示了一个轻便的协议, 利用这种技术, 在特定的地点, 在大肠杆菌细胞生成均匀乙酰蛋白。

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Cite this Article

Venkat, S., Gregory, C., Meng, K., Gan, Q., Fan, C. A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli. J. Vis. Exp. (130), e57061, doi:10.3791/57061 (2017).

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