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Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants

DOI:
10.3791/56771-v

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07:08 min

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March 06, 2018

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, , ,
1Department of Neurology,Johns Hopkins School of Medicine, 2Department of Biological Sciences,Delaware State University

Capitoli

  • 00:04Titolo
  • 01:31C. elegans Culturing and Synchronization
  • 03:11BPA Exposure
  • 04:27Anterior Touch Habituation Assay
  • 05:38Results: Fecundity and Adult Behavior After Early Embryonic Exposure to BPA
  • 06:14Conclusion

Summary

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Exposure to environmental toxicants can acutely impact development with long-term effects. Detailed protocols are provided to illustrate a strategy using an effective lab model to study the effect of early embryonic exposure to bisphenol A. We provide fecundity and behavioral assays to monitor the effectiveness of our toxicant exposure bio-assays.

Tags

C. ElegansBisphenol A (BPA)Embryonic ExposureLow-dose ToxicantsLong-term EffectsFecundityLearning BehaviorEndocrine Disrupting CompoundsDevelopmental EffectsBehavioral AssaysSynchronizationHypochlorite SolutionM9 Buffer

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