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Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
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Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
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November 17, 2017
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Summary
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Cell-autonomous functions of genes in the brain can be studied by inducing loss or gain of function in sparse populations of cells. Here, we describe in utero electroporation to deliver Cre recombinase into sparse populations of developing cortical neurons with floxed genes to cause loss of function in vivo.
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Bland, K. M., Casey, Z. O., Handwerk, C. J., Holley, Z. L., Vidal, G. S. Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation. J. Vis. Exp. (129), e56675, doi:10.3791/56675 (2017).
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