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Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
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Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
Capitoli
- 00:05Titolo
- 01:05Preparation of Pulled Pipettes
- 02:29In Utero Electroporation
- 06:54Brain Removal for Histological Preparation
- 08:06Results: Expression of GFP After Electroporation
- 09:18Conclusion
Cell-autonomous functions of genes in the brain can be studied by inducing loss or gain of function in sparse populations of cells. Here, we describe in utero electroporation to deliver Cre recombinase into sparse populations of developing cortical neurons with floxed genes to cause loss of function in vivo.
