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Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis
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Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis
Capitoli
- 00:04Titolo
- 00:50Single Cell Exocytosis Amperometry Experiments
- 04:00Intracellular Electrochemical Cytometry
- 06:47Results: The Effect of Osmotic Pressure on Exocytosis Activity
- 07:42Conclusion
Osmotic stress affects exocytosis and the amount of neurotransmitter released during this process. We demonstrate how combining electrochemical methods together with transmission electron microscopy can be used to study the effect of extracellular osmotic pressure on exocytosis activity, vesicle quantal size, and the amount of neurotransmitter released during exocytosis.
