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Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis
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JoVE Revista Neurociencias
Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis

Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis

8,360 Views

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08:08 min

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February 19, 2018

DOI:

10.3791/56537-v

DOI:
10.3791/56537-v

•

08:08 min
February 19, 2018

•

2 Views
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1Department of Chemistry and Chemical Engineering,Chalmers University of Technology, 2Department of Chemistry and Molecular Biology,University of Gothenburg

Summary

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Osmotic stress affects exocytosis and the amount of neurotransmitter released during this process. We demonstrate how combining electrochemical methods together with transmission electron microscopy can be used to study the effect of extracellular osmotic pressure on exocytosis activity, vesicle quantal size, and the amount of neurotransmitter released during exocytosis.

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Cite this Article

Fathali, H., Dunevall, J., Majdi, S., Cans, A. Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis. J. Vis. Exp. (132), e56537, doi:10.3791/56537 (2018).

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