Rivista
/
/
慢性活性泼斯坦-巴尔病毒感染患者 NK 和 T 细胞系建立的高效简便方法
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
DOI:

09:43 min

March 30, 2018

, , , , , ,

Capitoli

  • 00:04Titolo
  • 01:35Isolation of PBMC from CAEBV Patients
  • 02:59Expansion of Cells and Numbering the Viable Cells
  • 04:08Preparation for Cell Phenotyping by Flow Cytometry
  • 05:52Expansion and Cryopreservation of NK/T Cells
  • 07:23Results: Representative Gating Strategy for Flow Cytometry Analysis of Cell Lines L196 and M296
  • 08:25Conclusion

Summary

Traduzione automatica

采用高效、少量外周血、低剂量 IL-2 的方法, 建立了 CAEBV 患者的 NK 和 T 细胞克隆的简便算法。

Video correlati

Read Article