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An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
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09:43 min
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March 30, 2018
DOI:
1Genetic Resources Center, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences, 2MOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, 3State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences
Summary
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A simple method for obtaining NK and T cell clones from CAEBV patients was developed with high efficiency, a small amount of peripheral blood, and a low-dose of IL-2.
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Xu, C., Ai, J., Zhang, Q., Li, T., Wu, X., Xie, Z., Duan, Z. An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection. J. Vis. Exp. (133), e56515, doi:10.3791/56515 (2018).
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