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Biotinylated celle-gennemtrængende peptider til at studere intracellulær Protein-protein interaktioner
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Biochimica
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JoVE Journal Biochimica
Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Biotinylated celle-gennemtrængende peptider til at studere intracellulær Protein-protein interaktioner

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10:26 min

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December 20, 2017

DOI:

10.3791/56457-v

DOI:
10.3791/56457-v

•

10:26 min
December 20, 2017

•

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1Instituto de Neurociencias de Castilla y León (INCYL), Departamento de Bioquímica y Biología Molecular,Universidad de Salamanca, 2Centre for Cancer Research & Cell Biology (CCRCB), School of Medicine, Dentistry and Biomedical Sciences,Queen’s University Belfast

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Biotinylated Cell-penetrating PeptidesProtein-protein InteractionsIntracellular ContextBiotin-avidin Pull-down SystemSelf-penetrating SequencesCell SignalingG166 Glioma Stem CellsGSCsBCPPNeutrAvidinWestern Blot

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Jaraíz-Rodríguez, M., González-Sánchez, A., García-Vicente, L., Medina, J. M., Tabernero, A. Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions. J. Vis. Exp. (130), e56457, doi:10.3791/56457 (2017).

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