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A Simple Approach to Induce Experimental Autoimmune Neuritis in C57BL/6 Mice for Functional and Neuropathological Assessments
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
A Simple Approach to Induce Experimental Autoimmune Neuritis in C57BL/6 Mice for Functional and Neuropathological Assessments

A Simple Approach to Induce Experimental Autoimmune Neuritis in C57BL/6 Mice for Functional and Neuropathological Assessments

9,025 Views

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07:30 min

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November 09, 2017

DOI:

10.3791/56455-v

DOI:
10.3791/56455-v

•

07:30 min
November 09, 2017

•

7 Views
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1Department of Anatomy and Neuroscience, School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences,The University of Melbourne

Summary

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This report outlines a simple approach to successfully induce experimental autoimmune neuritis (EAN) using the myelin protein zero (P0)180-199 peptide in combination with Freund's complete adjuvant and pertussis toxin. We present a sophisticated paradigm capable of accurately assessing the extent of functional deficits and neuropathology that occur in this EAN.

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Cite this Article

Gonsalvez, D. G., Fletcher, J. L., Yoo, S. W., Wood, R. J., Murray, S. S., Xiao, J. A Simple Approach to Induce Experimental Autoimmune Neuritis in C57BL/6 Mice for Functional and Neuropathological Assessments. J. Vis. Exp. (129), e56455, doi:10.3791/56455 (2017).

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