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DNA 変更染色: 共焦点画像の数値解析
JoVE Journal
Genetica
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JoVE Journal Genetica
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
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DNA 変更染色: 共焦点画像の数値解析

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09:42 min

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September 07, 2017

DOI:

10.3791/56318-v

DOI:
10.3791/56318-v

•

09:42 min
September 07, 2017

•

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1Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences,University of Nottingham, 2School of Life Sciences Imaging (SLIM), School of Life Sciences,University of Nottingham, 3Children’s Brain Tumour Research Centre, School of Medicine, QMC,University of Nottingham

Summary

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新たに発見された 5-メチルシトシン (oxi mCs), 5-ヒドロキシメチルシトシン (5hmC), 5-formylcytosine (5 fC) の酸化フォームと 5-carboxylcytosine (5caC) は、独自の機能的役割の異なる DNA 変更を表す場合があります。ここの可視化から oxi mCs の空間分布、信号強度プロファイルとの共存の半定量的なワークフローを説明します。

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Cite this Article

Ramsawhook, A. H., Lewis, L. C., Eleftheriou, M., Abakir, A., Durczak, P., Markus, R., Rajani, S., Hannan, N. R., Coyle, B., Ruzov, A. Immunostaining for DNA Modifications: Computational Analysis of Confocal Images. J. Vis. Exp. (127), e56318, doi:10.3791/56318 (2017).

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