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Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
JoVE Journal
Genetik
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JoVE Journal Genetik
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

9,429 Views

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09:42 min

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September 07, 2017

DOI:

10.3791/56318-v

DOI:
10.3791/56318-v

•

09:42 min
September 07, 2017

•

3 Views
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1Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences,University of Nottingham, 2School of Life Sciences Imaging (SLIM), School of Life Sciences,University of Nottingham, 3Children’s Brain Tumour Research Centre, School of Medicine, QMC,University of Nottingham

Summary

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Newly discovered oxidized forms of 5-methylcytosine (oxi-mCs), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) may represent distinct DNA modifications with unique functional roles. Here a semi-quantitative workflow for visualization of oxi-mCs' spatial distribution, signal intensity profiling and colocalization is described.

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DNA ModificationsImmunostainingConfocal MicroscopyComputational AnalysisEpigeneticsNuclear LocalizationSignal Intensity2.5D VisualizationImage ProcessingZen Blue Software

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Cite this Article

Ramsawhook, A. H., Lewis, L. C., Eleftheriou, M., Abakir, A., Durczak, P., Markus, R., Rajani, S., Hannan, N. R., Coyle, B., Ruzov, A. Immunostaining for DNA Modifications: Computational Analysis of Confocal Images. J. Vis. Exp. (127), e56318, doi:10.3791/56318 (2017).

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