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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
JoVE Journal
Genetica
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JoVE Journal Genetica
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

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09:04 min

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September 21, 2017

DOI:

10.3791/56221-v

DOI:
10.3791/56221-v

•

09:04 min
September 21, 2017

•

5 Views
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1Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences,Nanyang Technological University

Summary

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We report the protocols for the synthesis and purification of Peptide Nucleic Acid (PNA) oligomers incorporating modified residues. The biochemical and biophysical methods for the characterization of the recognition of RNA duplexes by the modified PNAs are described.

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Toh, D. K., Patil, K. M., Chen, G. Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids. J. Vis. Exp. (127), e56221, doi:10.3791/56221 (2017).

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