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Stereological Estimation of Dopaminergic Neuron Number in the Mouse Substantia Nigra Using the Optical Fractionator and Standard Microscopy Equipment
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JoVE Journal Neuroscienze
Stereological Estimation of Dopaminergic Neuron Number in the Mouse Substantia Nigra Using the Optical Fractionator and Standard Microscopy Equipment

Stereological Estimation of Dopaminergic Neuron Number in the Mouse Substantia Nigra Using the Optical Fractionator and Standard Microscopy Equipment

DOI:
10.3791/56103-v

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08:19 min

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September 01, 2017

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, ,
1Department of Neurology,University Hospital of Würzburg

Capitoli

  • 00:05Titolo
  • 00:52Tissue Processing
  • 02:24Acquisition of Images
  • 04:20Sequence of Stereological Assessment
  • 06:37Results: Comparison of Cell Counting Parameters
  • 07:37Conclusion

Summary

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This work presents a step-by-step protocol for the unbiased stereological estimation of dopaminergic neuronal cell numbers in the mouse substantia nigra using standard microscopy equipment (i.e., a light microscope, a motorized object table (x, y, z plane), and public domain software for digital image analysis.

Tags

Stereological EstimationDopaminergic NeuronMouse Substantia NigraOptical FractionatorImmunohistochemistryParkinson’s DiseaseDesign-based StereologyCryosectioningTyrosine Hydroxylase

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