来到光明的一面: 体内糖尿病无毛小鼠慢性伤口生物膜感染的监测

JoVE Journal
Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model

来到光明的一面: 体内糖尿病无毛小鼠慢性伤口生物膜感染的监测

DOI:

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09:15 min

October 10, 2017

00:05Titolo
01:13Diabetes Induction and Surgical Preparation
03:12Wound Surgery
04:36Biofilm Inoculum Infection, Bioluminescence Expression Assessment, and Wound Measurement and Imaging
07:04Results: Representative Bioluminescence Expression, Biofilm Evolution, and Wound Closure Analyses
08:08Conclusion

在这里, 我们描述了一个新的糖尿病小鼠模型利用无毛 real-time, 无创, 监测生物膜伤口感染生物发光的铜绿假单胞菌。该方法可用于评价其他细菌种类和转基因微生物的感染, 包括多生物膜, 并对 antibiofilm 策略的有效性进行测试。