Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model
12,953 Views
•
09:15 min
•
October 10, 2017
DOI:
Summary
Automatically generated
Here we describe a novel diabetic murine model utilizing hairless mice for real-time, non-invasive, monitoring of biofilm wound infections of bioluminescent Pseudomonas aeruginosa. This method can be adapted to evaluate infection of other bacterial species and genetically modified microorganisms, including multi-species biofilms, and test the efficacy of antibiofilm strategies.
Read Article
Cite this Article
Hunt, A. M. A., Gibson, J. A., Larrivee, C. L., O'Reilly, S., Navitskaya, S., Busik, J. V., Waters, C. M. Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model. J. Vis. Exp. (128), e55991, doi:10.3791/55991 (2017).
Copy