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Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
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Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
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06:45 min
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February 28, 2019
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We describe the detailed procedures and strategies to measure the mechanical properties and mechanical unfolding pathways of single protein molecules using an atomic force microscope. We also show representative results as a reference for selection and justification of good single protein molecule recordings.
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Scholl, Z. N., Li, Q., Josephs, E., Apostolidou, D., Marszalek, P. E. Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope. J. Vis. Exp. (144), e55989, doi:10.3791/55989 (2019).
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