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Chemistry

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In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS
 

In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS

Article DOI: 10.3791/55944-v • 09:56 min • August 18th, 2017
August 18th, 2017 •
1, 1, 2, 1, 1, 2, 1
1Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, 2Wiley Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory

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This article presents a method for growing a biofilm for in situ time-of-flight secondary ion mass spectrometry for chemical mapping in its hydrated state, enabled by a microfluidic reactor, System for Analysis at the liquid Vacuum Interface. The Shewanella oneidensis MR-1 with green fluorescence protein was used as a model.

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SALVI ToF-SIMS Shewanella Oneidensis MR1 Biofilms In Situ Characterization Imaging Mass Spectrometry Visual Demonstration Tubing Setup Biosafety Cabinet PTFE Tubing
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