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JoVE Journal Neuroscience
Tuning in the Hippocampal Theta Band In Vitro
 

Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit

Article DOI: 10.3791/55851-v • 11:37 min • August 2nd, 2017
August 2nd, 2017 •
1, 1
1Department of Psychiatry, Douglas Mental Health University Institute, McGill University

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Here, we present a protocol for recording rhythmic neuronal network theta and gamma oscillations from an isolated whole hippocampal preparation. We describe the experimental steps from extraction of the hippocampus to details of field, unitary and whole-cell patch clamp recordings as well as optogenetic pacing of the theta rhythm.

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Hippocampal Theta Band In Vitro Recording Isolated Rodent Septohippocampal Circuit Field Recording Unitary Recording Patch-clamp Recording Optogenetic Stimulation Neuroscience Learning And Memory Cellular Mechanisms Synaptic Mechanisms Oscillations Memory Information Brain Dissection Cerebellum Removal Brain Hemispheres Holding Chamber Sagittal Structures Septal Complex Tissue Dissection
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