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Genoom-wide mapping van eiwit-DNA-interacties met ChEC-seq in<em> Saccharomyces cerevisiae</em
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Genetica
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JoVE Journal Genetica
Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
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Genoom-wide mapping van eiwit-DNA-interacties met ChEC-seq in<em> Saccharomyces cerevisiae</em

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10:43 min

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June 03, 2017

DOI:

10.3791/55836-v

DOI:
10.3791/55836-v

•

10:43 min
June 03, 2017

•

1 Views
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1Basic Sciences Division,Fred Hutchinson Cancer Research Center, 2Department of Biology,Indiana University

Summary

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We beschrijven chromatin endogene splitsing gekoppeld aan high-throughput sequencing (ChEC-seq), een chromatine immunoprecipitatie (ChIP) -orthogonale methode voor het mappen van eiwitbindingsplaatsen genoom-wijd met micrococculaire nuclease (MNase) fusie-eiwitten.

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Cite this Article

Grünberg, S., Zentner, G. E. Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae. J. Vis. Exp. (124), e55836, doi:10.3791/55836 (2017).

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