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从人体二尖瓣提取蛋白质的优化方案
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Biochimica
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JoVE Journal Biochimica
Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
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从人体二尖瓣提取蛋白质的优化方案

12,679 Views

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09:13 min

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June 14, 2017

DOI:

10.3791/55762-v

DOI:
10.3791/55762-v

•

09:13 min
June 14, 2017

•

7 Views
, , , , , ,
1Centro Cardiologico Monzino IRCCS, 2Cardiovascular Tissue Bank of Milan,Centro Cardiologico Monzino IRCCS, 3Department of Clinical Sciences and Community Health, Cardiovascular Section,University of Milan, 4Department of Cardiovascular Disease, Development and Innovation Cardiac Surgery Unit,Centro Cardiologico Monzino IRCCS

Summary

Automatically generated

人二尖瓣的蛋白质组成仍然是部分未知的，因为其分析由于细胞数量低，因此通过低蛋白质生物合成而复杂化。这项工作提供了有效提取蛋白质用于二尖瓣蛋白质组分析的方案。

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Cite this Article

Banfi, C., Guarino, A., Brioschi, M., Ghilardi, S., Mastrullo, V., Tremoli, E., Polvani, G. Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve. J. Vis. Exp. (124), e55762, doi:10.3791/55762 (2017).

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