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Studie af Cyklusreguleret Gen Expression af To Komplementære Celle Synkronisering Protokoller
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JoVE Journal Genetica
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studie af Cyklusreguleret Gen Expression af To Komplementære Celle Synkronisering Protokoller

26,576 Views

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12:02 min

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June 06, 2017

DOI:

10.3791/55745-v

DOI:
10.3791/55745-v

•

12:02 min
June 06, 2017

•

11 Views
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1Department of Cell Biology and Histology,University of the Basque Country, UPV/EHU, 2Department of Genetics, Physical Anthropology and Animal Physiology,University of the Basque Country, UPV/EHU, 3Department of Molecular Mechanisms of Disease,University of Zurich

Summary

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Vi rapporterer to cellesynkroniseringsprotokoller, der giver en sammenhæng til at studere begivenheder relateret til specifikke faser af cellecyklussen. Vi viser, at denne tilgang er nyttig til analyse af reguleringen af ​​specifikke gener i en upertureret cellecyklus eller ved udsættelse for midler, som påvirker cellecyklussen.

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Apraiz, A., Mitxelena, J., Zubiaga, A. Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols. J. Vis. Exp. (124), e55745, doi:10.3791/55745 (2017).

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