This work presents a method to quickly and precisely quantify the abdominal pigmentation of Drosophila melanogaster using digital image analysis. This method streamlines the procedures between phenotype acquisition and data analysis and includes specimen mounting, image acquisition, pixel value extraction, and trait measurement.
Saleh Ziabari, O., Shingleton, A. W. Quantifying Abdominal Pigmentation in Drosophila melanogaster. J. Vis. Exp. (124), e55732, doi:10.3791/55732 (2017).