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Quantifying Abdominal Pigmentation in Drosophila melanogaster
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JoVE Journal Genetica
Quantifying Abdominal Pigmentation in Drosophila melanogaster

Quantifying Abdominal Pigmentation in Drosophila melanogaster

DOI:
10.3791/55732-v

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08:41 min

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June 01, 2017

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1Department of Biology,Lake Forest College

Capitoli

  • 00:05Titolo
  • 00:53Visualizing the Specimen
  • 04:18Image Analysis
  • 06:44Results: Testing the Methodology
  • 08:07Conclusion

Summary

Traduzione automatica

Traduzione automatica

This work presents a method to quickly and precisely quantify the abdominal pigmentation of Drosophila melanogaster using digital image analysis. This method streamlines the procedures between phenotype acquisition and data analysis and includes specimen mounting, image acquisition, pixel value extraction, and trait measurement.

Tags

Keywords: Abdominal PigmentationDrosophila MelanogasterEvolutionary BiologyMorphological PhenotypesNon-destructive MeasurementPigmentation PatternsInsectsAnimal TaxaMounting PadAgar GelMicroscope ImagingDigital CameraImage Analysis SoftwareGrayscaleLight SourceStage MicrometerScale Calibration

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