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Proteïne-tRNA Agarose Gel Retardatie Assays voor de analyse van de<em> N</em<sup> 6</sup> -threonylcarbamoyladenosine TcdA-functie
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Biochimica
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JoVE Journal Biochimica
Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function
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Proteïne-tRNA Agarose Gel Retardatie Assays voor de analyse van de<em> N</em<sup> 6</sup> -threonylcarbamoyladenosine TcdA-functie

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08:03 min

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June 21, 2017

DOI:

10.3791/55638-v

DOI:
10.3791/55638-v

•

08:03 min
June 21, 2017

•

10 Views
, , , ,
1Department of Physical Chemical Biology, Center for Biological Research (CIB-CSIC),Spanish National Research Council (CSIC), 2Department of Immunology,Complutense University School of Medicine, 3Abvance Biotech srl

Summary

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Een protocol wordt gepresenteerd voor de productie van tRNA (UUU) en de analyse van tRNA (UUU) in complex met het enzym TcdA door middel van agarose gel retardatie assays.

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Fernández, F. J., Gómez, S., Navas-Yuste, S., López-Estepa, M., Vega, M. C. Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function. J. Vis. Exp. (124), e55638, doi:10.3791/55638 (2017).

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