This paper describes the assembly process and operation of a bench-scale photosynthetic bioreactor that can be used, in conjunction with other methods, to estimate pertinent kinetic growth parameters. This system continuously monitors the pH, light, and temperature using sensors, a data acquisition and control unit, and open-source data acquisition software.
Karam, A. L., McMillan, C. C., Lai, Y., de los Reyes III, F. L., Sederoff, H. W., Grunden, A. M., Ranjithan, R. S., Levis, J. W., Ducoste, J. J. Construction and Setup of a Bench-scale Algal Photosynthetic Bioreactor with Temperature, Light, and pH Monitoring for Kinetic Growth Tests. J. Vis. Exp. (124), e55545, doi:10.3791/55545 (2017).