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斑马鱼<em>原位</em>脊髓制备用于脊髓感觉和运动神经元的电生理记录

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons

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斑马鱼<em>原位</em>脊髓制备用于脊髓感觉和运动神经元的电生理记录

DOI:

10.3791/55507-v

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08:24 min

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April 18, 2017

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Rosa L. Moreno, Megan Josey, Angeles B. Ribera²

¹Department of Physiology and Biophysics,University of Colorado Anschutz Medical Campus (UCAMC), ²Neuroscience Graduate Program,University of Colorado Anschutz Medical Campus (UCAMC)

Capitoli

00:05Titolo
00:48Preparation of Disseciton Materials
02:32Dissection of Embryo for Patch-clamp Recordings from Spinal Neurons
07:00Results: Excitability Properties of Bohon-Beard Neurons
07:51Conclusion

Summary

Traduzione automatica

这手稿描述了从斑马鱼胚胎和幼虫的脊髓神经元的电生理记录的方法。制备维持神经元在原地 ，往往涉及到最小的清扫。这些方法允许对多种脊髓神经元的电生理学研究中，从通过早期幼体阶段的初始电兴奋采集。

Tags

Keywords: Zebrafish In Situ Spinal Cord Electrophysiology Spinal Neurons Sensory Neurons Motor Neurons Embryo Larva Rohon Beard Neurons Silicone Elastomer Dissection Chamber Ringer’s Solution Tricaine Micropipette

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