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Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons
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Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons
Chapitres
- 00:05Titre
- 00:48Preparation of Disseciton Materials
- 02:32Dissection of Embryo for Patch-clamp Recordings from Spinal Neurons
- 07:00Results: Excitability Properties of Bohon-Beard Neurons
- 07:51Conclusion
This manuscript describes methods for electrophysiological recordings from spinal neurons of zebrafish embryos and larvae. The preparation maintains neurons in situ and often involves minimum dissection. These methods allow for the electrophysiological study of a variety of spinal neurons, from the initial electrical excitability acquisition through the early larval stages.
