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对酶活性G4 Resolvase1净化用G-四DNA亲和方法
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March 18, 2017
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G4 Resolvase1结合G-四（G4）的结构与一个G4结合蛋白的紧密报道的亲和力，并表示在HeLa细胞中的大多数G4-DNA解旋活动。我们描述了线束的亲和力和G4-Resolvase1的ATP依赖的平仓活动，特别是净化催化活性的重组G4R1一个新的协议。
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Routh, E. D., Creacy, S. D., Beerbower, P. E., Akman, S. A., Vaughn, J. P., Smaldino, P. J. A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1. J. Vis. Exp. (121), e55496, doi:10.3791/55496 (2017).
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