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支气管肺泡灌洗小鼠肺分析炎症细胞浸润
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration
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支气管肺泡灌洗小鼠肺分析炎症细胞浸润

DOI:
10.3791/55398-v

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07:03 min

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May 04, 2017

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, , ,
1UGent Center for Medical Biotechnology,VIB, 2Department of Biomedical Molecular Biology,Ghent University (UGent)

Capitoli

  • 00:05Titolo
  • 00:52Broncoalveolar Lavage (BAL)
  • 02:58BAL Fluid Component Collection and Immunostaining for Flow Cytometry (FACS)
  • 04:27Results: Representative BAL Immune Cell Detection
  • 06:17Conclusion

Summary

Traduzione automatica

Traduzione automatica

肺的健康状况的类型和存在于肺的细支气管的免疫细胞的数目反射。我们描述了支气管肺泡灌洗技术，它允许从小鼠的下呼吸道隔离和非粘附细胞和可溶性因子的研究。

Tags

Bronchoalveolar LavageMurine LungsInflammatory Cell InfiltrationRespiratory ImmunologyLung InfectionLung InflammationInnate Cellular ResponseHumoral ResponseBAL FluidCell HarvestingEx Vivo AnalysisDisease Assessment

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