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Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration

Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration

DOI:

07:03 min

May 04, 2017

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Chapters

  • 00:05Title
  • 00:52Broncoalveolar Lavage (BAL)
  • 02:58BAL Fluid Component Collection and Immunostaining for Flow Cytometry (FACS)
  • 04:27Results: Representative BAL Immune Cell Detection
  • 06:17Conclusion

Summary

自动翻译

The health status of the lung is reflected by the type and number of immune cells that are present in the bronchioles of the lung. We describe a bronchoalveolar lavage technique that allows the isolation and study of nonadherent cells and soluble factors from the lower respiratory tract of mice.

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