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Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

JoVE Journal
Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

6,503 Views

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10:18 min

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June 26, 2017

DOI:

10.3791/55297-v

DOI:

10.3791/55297-v

•

10:18 min

June 26, 2017

•

14 Views

Lorena Benedetti^2,3, Anna Ghilardi, Laura Prosperi, Maura Francolini*¹, Luca Del Giacco*⁴

¹Department of Medical Biotechnology and Translational Medicine,Università degli Studi di Milano, ²Department of Neuroscience; Department of Cell Biology,Howard Hughes Medical Institute, Yale University School of Medicine, ³Program in Cellular Neuroscience, Neurodegeneration and Repair,Yale University School of Medicine, ⁴Department of BioSciences,Università degli Studi di Milano

Tags

Biosensing Motor Neuron Membrane Potential Live Zebrafish Embryos FRET-based GEVI Noninvasive Excitable Cells Neurons Neurological Disorders Mermaid Biosensor HuC Promoter PCR Restriction Digestion DNA Ligation Transformation

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