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Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation

Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation

DOI:

10:15 min

March 22, 2017

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Capitoli

  • 00:05Titolo
  • 01:213T3 Feeder Cell Culture and Irradiation
  • 02:31Pediatric Esophageal Biospy Acquisition and Processing
  • 05:00Cell Culture Expansion, Freezing, and Storage
  • 07:17Results: Representative Human Esophageal Cell Characterization
  • 08:59Conclusion

Summary

Traduzione automatica

Expansion of human pediatric esophageal epithelial cells utilizing conditional reprogramming provides investigators with a patient-specific population of cells that can be utilized for engineering esophageal constructs for autologous implantation to treat defects or injury and serve as a reservoir for therapeutic screening assays.

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