A simple red blood cell lysis method for establishing B-LCLs was developed with high immortalization efficiency, requiring a small amount of blood and saving time from initiation to cryopreservation.
Liu, X., Xu, C., Duan, Z. A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines. J. Vis. Exp. (119), e55191, doi:10.3791/55191 (2017).