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A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
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JoVE 杂志 免疫与感染
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines

A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines

14,649 Views

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09:49 min

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January 14, 2017

DOI:

10.3791/55191-v

DOI:
10.3791/55191-v

•

09:49 min
January 14, 2017

•

1 Views
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1Wuhan Institute of Virology,Chinese Academy of Sciences, 2Genetic Resources Center, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences

Summary

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A simple red blood cell lysis method for establishing B-LCLs was developed with high immortalization efficiency, requiring a small amount of blood and saving time from initiation to cryopreservation.

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B Lymphoblastoid Cell LinesB-LCLsImmortalization EfficiencyRed Blood Cell LysisEpstein-Barr VirusPBMCDensity Gradient CentrifugationEBV TransformationCell CultureCryopreservationVirus Particle ReleaseFiltrationWhole Blood

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Cite this Article

Liu, X., Xu, C., Duan, Z. A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines. J. Vis. Exp. (119), e55191, doi:10.3791/55191 (2017).

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