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Bリンパ芽球様細胞株の樹立のためのシンプルな赤血球溶解法
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
DOI:

09:49 min

January 14, 2017

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Capitoli

  • 00:05Titolo
  • 01:36Preparation of Epstein Barr Virus
  • 03:32Treatment of Whole Blood with Red Blood Cell Lysis Buffer and Epstein Barr Virus Infection
  • 05:29Expansion and Cryopreservation of B-LCLs
  • 07:27Results: B Lymphoblastoid Cell Viability Following Red Blood Cell Lysis
  • 08:35Conclusion

Summary

Traduzione automatica

B-LCLを確立するための単純な赤血球溶解方法は、少量の血液を必要とし、凍結保存し、開始からの時間を節約し、高い不死化効率で開発されました。

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