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Metagenomic Analysis of Silage
JoVE Journal
Genetica
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JoVE Journal Genetica
Metagenomic Analysis of Silage

Metagenomic Analysis of Silage

DOI:
10.3791/54936-v

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08:43 min

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January 13, 2017

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, , , ,
1Biosciences,University of Exeter

Capitoli

  • 00:05Titolo
  • 00:55DNA Extraction
  • 03:12DNA Purification
  • 04:23Quantification of Purified DNA
  • 05:14Shotgun Sequencing Library Preparation
  • 06:28Results: Characterization of the Microbial Community Present in Cattle Silage
  • 07:38Conclusion

Summary

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Metagenomics was used to investigate the microbiome of silage cattle feed. Analysis was performed by shotgun sequencing. This approach was used to characterize the composition of the microbial community within the cattle feed.

Tags

MetagenomicsSilageBacterial CommunityMicrobial AnalysisDNA ExtractionShotgun SequencingMicrobiomeLysisCentrifugationDNA Binding MatrixWash BufferDNA Elution

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