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Immunologia e infezioni

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Un protocolo optimizado para analizar la glucólisis y respiración mitocondrial en los linfocitos

JoVE Journal
Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes

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Un protocolo optimizado para analizar la glucólisis y respiración mitocondrial en los linfocitos

28,872 Views

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08:40 min

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November 21, 2016

DOI:

10.3791/54918-v

DOI:

10.3791/54918-v

•

08:40 min

November 21, 2016

•

4 Views

Javier Traba*¹, Pietro Miozzo*², Billur Akkaya, Susan K. Pierce, Munir Akkaya

¹Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute,National Institutes of Health, ²Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases,National Institutes of Health, ³Laboratory of Immunology, National Institute of Allergy and Infectious Diseases,National Institutes of Health

Tags

Glycolysis Mitochondrial Respiration Lymphocytes Extracellular Flux Assay Immunometabolism Metabolic Profiles Non-adherent Cells Cell Functionality Cell Viability Cell Strainer ACK Red Blood Cell Lysis Buffer MS Buffer Cell Suspension Cell Pellet Centrifugation

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