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An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
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An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
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08:40 min
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November 21, 2016
DOI:
1Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute,National Institutes of Health, 2Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 3Laboratory of Immunology, National Institute of Allergy and Infectious Diseases,National Institutes of Health
Summary
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The study of metabolism is becoming increasingly relevant to immunological research. Here, we present an optimized method for measuring glycolysis and mitochondrial respiration in mouse splenocytes, and T and B lymphocytes.
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Traba, J., Miozzo, P., Akkaya, B., Pierce, S. K., Akkaya, M. An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes. J. Vis. Exp. (117), e54918, doi:10.3791/54918 (2016).
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