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リンパ球における解糖およびミトコンドリア呼吸を分析するために最適化されたプロトコル
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
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リンパ球における解糖およびミトコンドリア呼吸を分析するために最適化されたプロトコル

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08:40 min

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November 21, 2016

DOI:

10.3791/54918-v

DOI:
10.3791/54918-v

•

08:40 min
November 21, 2016

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1Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute,National Institutes of Health, 2Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 3Laboratory of Immunology, National Institute of Allergy and Infectious Diseases,National Institutes of Health

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GlycolysisMitochondrial RespirationLymphocytesExtracellular Flux AssayImmunometabolismMetabolic ProfilesNon-adherent CellsCell FunctionalityCell ViabilityCell StrainerACK Red Blood Cell Lysis BufferMS BufferCell SuspensionCell PelletCentrifugation

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Traba, J., Miozzo, P., Akkaya, B., Pierce, S. K., Akkaya, M. An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes. J. Vis. Exp. (117), e54918, doi:10.3791/54918 (2016).

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